Table 1.

Primer and probe sequences and concentrations for qPCR

Gene name	Sequence	Used concentration (nm)
KCNMA1 (α)
Forward primer 5′–3′	CTCCAATGAAATGTACACAGAATATCT	300
Reverse primer 3′–5′	CTATCATCAGGAGCTTAAGCTTCACA	300
Probe	CCTTCGTGGGTCTGTCCTTCCCTACTGTT	200
KCNMB1 (β1)
Forward primer 5′–3′	TAGTGTGTGCTGCCATCACC	100
Reverse primer 3′–5′	GCTCTTCCTGGTCCTTGATATTAG	300
Probe	CGTCCTGGGTACAACTGTGCTGCC	300
KCNMB2 (β2)
Forward primer 5′–3′	TGCAGGACCAACATCCTCTAAG	300
Reverse primer 3′–5′	CTTCAGAGCTGTCACAGTTTTCC	300
Probe	TGGACCAGTGGCCGGACCTCTTCA	100
KCNMB3 (β3)
Forward primer 5′–3′	ACTCGGGACAGAAAGTGCTTC	500
Reverse prime 3′–5′r	GATGTCCAGGACGCTGTTGAG	300
Probe	ACGACGACGAGGCCATCAGGACCA	300
KCNMB4 (β4)
Forward primer 5′–3′	CAACTCCGAGTCCAACTCCAG	300
Reverse primer 3′–5′	TCTCTTACAGGGCGGGATATAGG	100
Probe	ACACAGCGACCAGCACCAGCTCCT	200
CHIF (corticosteroid hormone-induced factor)
Forward primer 5′–3′	TGACCCAGTTGATAAAGACAGTCC	300
Reverse primer 3′–5′	CATTTGCACTTGCCACTCAGG	300
Probe	TTCCAGCGATGCACAGGAGCCCTC	200
β-Actin
Forward primer 5′–3′	TTTGCAGCTCCTTCGTTGCC	100
Reverse primer 3′–5′	CACGATGGAGGGGAATACAGC	500
Probe	TCCACACCCGCCACCAGTTCGCC	300
HPRT
Forward primer 5′–3′	CTTCCTCCTCAGACCGCTTTT	300
Reverse primer 3′–5′	TCTAGGTCATAACCTGGTTCATCA	300
Probe	CGCTAATCACGACGCTGGGACTGC	300