FIG. 2.

Hemodynamic responses for total blood volume (Hbt), oxyhemoglobin (HbO₂) and deoxyhemoglobin (Hbr) for long duration stimulation of whiskers A1–E1. A: shows normalised mean response images for Hbt, HbO₂ and the inverse of Hbr taken from 12–16 s after stimulation onset. The most localized responses are from the Hbt data. B: combined surface and barrel histological sections with the stimulated barrel highlighted in black. The contour round each stimulated barrel is the activated Hbt region calculated by including all pixels with 50% of the peak response from a mean image of the last 4 s of stimulation. Scale bar = 500 μm.