Figure 5.

(A–C) Cells treated with SULF2 siRNA have an enhanced proliferation compared with cells treated with control siRNA. MCF-7B (A; Mao et al. 2005), MDA MD231 (B), and MCF-10A (C) cells were transfected with 50 nM SULF2 or control siRNA; 10⁴ cells were plated in medium containing 10% FBS 48 h after transfection of the siRNA. Cells were counted on day 2, 4, 6, and 8. Experiments performed in triplicate; error bars show standard deviation. (D–F) Cells treated with SULF2 siRNA have an enhanced survival compared with cells treated with control siRNA. MCF-7B (D; Mao et al. 2005), MDA MD231 (E), and MCF-10A (F) cells were transfected with 50 nM SULF2 or control siRNA; 10⁴ cells were plated in serum-free medium 48 h after transfection of the siRNA. Cells were counted on day 2, 4, and 6. Experiments performed in triplicate. Error bars, SD. (G,H) Treatment of MCF-7B and MDA MB231 cells with siRNA for SULF2 increases the anchorage-independent growth capabilities. After treatment with siRNA, 10⁴ cells were plated in 0.3% agar in growth medium, MCF-7B colony formation is shown in G. Plates were incubated for 21 d, and colonies were counted; bar chart results shown in H. Experiments performed in triplicate. Error bars, SD.