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. 2008 Jul 4;47(30):7875–7887. doi: 10.1021/bi800731m

Table 3. Kinetic Parameters for H115W−dNTP Binding.

  k1 (×106 M−1 s−1) k−1 (s−1) Kd (μM)a
dCTP 0.055 ± 0.001 0.51 ± 0.001 9.3 ± 0.2
dGTP 0.17 ± 0.002 0.12 ± 0.0004 0.71 ± 0.01
dATP 0.18 ± 0.003 0.11 ± 0.0005 0.61 ± 0.01
dTTP 0.050 ± 0.001 0.75 ± 0.002 15 ± 0.3
a

k1 was calculated as the slope of the linear fit of rate of dNTP binding as a function of dNTP concentration. k−1 was obtained from a single-exponential fit of the fluorescence change upon dNTP release in the presence of 20 mM EDTA. KD was calculated as k−1/k1.