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Imino proton regions of 1D jump–return echo spectra acquired at 10 °C and 10 mM KPi (pH 6.0) for WT* (a), ΔA35 (b), ΔA35/U33C (c), ΔA35/C34U (d), and ΔA35/A36U (e). Imino protons corresponding to noncanonical base pairs are shown in bold. Note that some spectra (WT*, ΔA35/U33C, and ΔA35/C34U) are characterized by slow conformational heterogeneity at the base portion of SL1 (A5-U40, G6-C39, and A7-U38 base pairs).
