FIGURE 2.

A, organization of hp310 gene and surrounding genes in H. pylori genome and DNA constructs for generating the HP310 mutant. A fragment containing the hp310 gene was PCR-amplified using the F- and R-primer pair and cloned into pGEM-T to obtain pGEM-hp310. A kanamycin resistance cassette (Kan) was then inserted within the HP310 fragment at the BamHI site, and the plasmid pGEM-hp310:Kan was used for transformation of wild type H. pylori to create the hp310:kan mutant. B, amino acid sequence alignment. The N-terminal portion of HP310 (residues 44–90, equivalent to the region marked with gray bar in A) shows a limited homology to the C-terminal catalytic domain (residues 284–330) of S. pneumoniae PgdA (peptidoglycan deacetylase). The two catalytic histidine residues are in bold.