TABLE 2.
Comparison of the muropeptides of H. pylori WT and HP310 mutant by MALDI-TOF analysis
|
Muropeptidea
|
Peak no.
|
Observed
ionb
|
|
|---|---|---|---|
| Parent strain | HP310:Kan | ||
| 894 | 894 | ||
| 965 | 965 | ||
| 979 | 981 | ||
| 1 | 993 | 993 | |
| 2 | 1015 | 1015 | |
| 1021 | 1022 | ||
| Tetra-G-G | 1036 | 1037 | |
| 1052 | 1053 | ||
| 3 | 1196 | ||
| 4 | 1399 | ||
| 5 | 1713 | ||
| 6 | 1896 | 1898 | |
| 1917 | 1920 | ||
| Tetra-G-G-tetra | 1937 | 1939 | |
| 1955 | 1957 | ||
| 7 | 2009 | ||
| 8 | 2026 | ||
a
Tetra = disaccharide-tetrapeptide (Ala-Glu-Lys-Ala); disaccharide = GlcNAc-MurNAc, reduced with sodium borodeuteride; G = Gly; assignments represent the most likely fit based on typical amino acid components.
b
Ions are present as the sodiated molecular species, [M + Na]+1, rounded to nearest whole mass.