FIGURE 6.
PtdCho biosynthesis and IgM secretion in vitro by WT and KO B-cells in response to LPS. A, the rate of incorporation of [3H]choline into PtdCho was determined before and at 24 or 48 h after the addition of LPS to KO (dark gray) or WT (light gray) purified splenic B-cells. B, the amount of IgM secreted in vitro during a 1-h period from KO (dark gray) or WT (light gray) B-cells was determined before and 24 or 48 h after stimulation with LPS. The data are the mean ± S.E. from at least three independent determinations in two independent experiments.