TABLE 3.

Thermodynamic parameters of interaction of isolated CBM40 from V. cholerae sialidase with different linkage sialosides

Sialoside	Linkage specificity	n	ΔH	TΔS	ΔG	Ka	Kd
			kcal/mol	kcal/mol	kcal/mol	10−4m	μm
3′SL	α2,3	0.96 ± 0.002	−16.3 ± 0.05	−9.8	−6.5	5.48 ± 0.05	18
6′SL	α2,6	1.02 ± 0.001	−9.9 ± 0.002	−3.5	−6.4	5.16 ± 0.03	19
DSL	α2,8 α2,3	0.92 ± 0.001	−12.5 ± 0.003	−5.7	−6.8	10.2 ± 0.01	9.8
DSLNT	α2,6, α2,3	0.57 ± 0.001	−24.0 ± 0.007	−17.3	−6.7	7.8 ± 0.06	13