TABLE 5.

Biacore kinetic parameters for the different CBM polypeptides interacting with multivalent 3′SL (n = 3 determinations)

Peptide	T	ka	kd	KD
		m−1 s−1	s−1	m
1CBM	35	(3.1 ± 0.05) × 103	(1.8 ± 0.3) × 10−2	5.8 × 10−6
	25	(4.3 ± 0.7) × 103	(7.6 ± 0.4) × 10−3	1.8 × 10−6
	15	(3.7 ± 0.4) × 103	(3.6 ± 0.9) × 10−3	9.7 × 10−7
2CBM(5)	35	(3.5 ± 0.2) × 105	(3.6 ± 0.04) × 10−2	1.0 × 10−7
	25	(5.6 ± 0.7) × 105	(2.5 ± 0.1) × 10−2	4.5 × 10−8
	15	(7.4 ± 0.13) × 105	(9.5 ± 0.07) × 10−3	1.3 × 10−8
2CBM(10)	35	(2.0 ± 0.1) × 104	(2.6 ± 0.2) × 10−3	1.3 × 10−7
	25	(5.9 ± 0.3) × 105	(2.3 ± 0.23) × 10−2	3.9 × 10−8
	15	(8.1 ± 0.17) × 105	(6.9 ± 0.05) × 10−3	8.5 × 10−9
2CBM(15)	35	(5.3 ± 0.6) × 105	(5.2 ± 0.3) × 10−2	9.8 × 10−8
	25	(8.8 ± 0.5) × 105	(3.4 ± 0.08) × 10−2	3.8 × 10−8
	15	(8.7 ± 0.5) × 105	(8.7 ± 0.3) × 10−3	1 × 10−8
3CBM(5)	35	(5.2 ± 0.35) × 105	(4.4 ± 0.2) × 10−3	8.44 × 10−9
	25	(5.6 ± 0.15) × 105	(2.3 ± 0.08) × 10−3	4.0 × 10−9
	15	(3.0 ± 0.3) × 105	(1.1 ± 0.04) × 10−3	3.7 × 10−9
3CBM(10)	35	(5.1 ± 0.01) × 105	(4.1 ± 0.48) × 10−3	8.07 × 10−9
	25	(4.5 ± 0.4) × 105	(1.95 ± 0.03) × 10−3	4.26 × 10−9
	15	(3.3 ± 0.2) × 105	(1.21 ± 0.02) × 10−3	3.63 × 10−9
4CBM(5)	35	(2.2 ± 0.8) × 105	(9.2 ± 0.07) × 10−4	4.01 × 10−9
	25	(2.8 ± 0.5) × 105	(7.4 ± 0.9) × 10−4	2.62 × 10−9
	15	(2.9 ± 0.4) × 105	(2.5 ± 0.01) × 10−4	8.61 × 10−10