Lpin2 gene expression is increased by food deprivation but is not a
target of PGC-1α. A, the graph represents mean hepatic
expression of Lpin1 or Lpin2 in fasted or re-fed mice. *,
p < 0.05 versus fasted control. Inset at top,
representative Western blots showing expression of lipin 2 and actin protein.
AU, arbitrary units. B, the graph represents mean hepatic
expression of Lpin2 in fasted or re-fed WT or
PGC-1α–/– mice. *, p < 0.01
versus fasted control. C, the graph represents mean
expression of Lpin1 or Lpin2 in primary hepatocytes
overexpressing PGC-1α and/or green fluorescent protein (GFP,
control) as quantified by quantitative reverse transcription-PCR 48 h after
infection. *, p < 0.01 versus green fluorescent protein
control. Inset at top, representative Western blots showing
expression of lipin 2 and PGC-1α protein.