TABLE 2.
Yeast genes isolated from the synthetic genetic array screen whose inactivation aggravated growth of sec14ts cells
| Gene | Open reading frame | Description | Cellular process |
|---|---|---|---|
| SPO14 | YKR031C | Phospholipase D, catalyzes hydrolysis of phosphatidylcholine to phosphatidic acid and choline | Vesicular transport/phospholipid metabolism |
| YPT31 | YER031C | GTPase of Rab family involved in the endocytic and exocytic pathways | Vesicular transport |
| TRS33 | YOR115C | Protein of the TRAPPII complex involved in endocytic and exocytic pathways; GEF activity toward Ypt31 | Vesicular transport |
| GYP1 | YOR070C | Golgi GTPase-activating protein for Rab family members | Vesicular transport |
| TLG2 | YOL018C | t-SNARE involved in endocytosis and maintenance of resident proteins in the trans-Golgi | Vesicular transport |
| UBP3 | YER151C | Ubiquitin-specific protease, interacts with Bre5 | Vesicular transport |
| BRE5 | YNR051C | Required by Ubp3 to form an active de-ubiquitination complex which protects Sec23, a COPII subunit, from degradation | Vesicular transport |
| KEX2 | YNL238W | Calcium-dependent serine protease involved in the activation of proproteins of the secretory pathway | Vesicular transport/Golgi function |
| VPS1 | YKR001C | Dynamin-like GTPase for vacuolar protein sorting and high density vesicle production for secretion of invertase | Vesicular transport |
| TCB2 | YNL087W | Synaptotagmin like protein; contains 3 C2 domains | Golgi localized |
| INP51 | YIL002C | PI-4,5-bisphosphate 5-phosphatase | Phospholipid metabolism |
| YBR030W | YBR030W | Protein of unknown function. Likely involved in phospholipid metabolism as it has Ino2/4-binding sites in promoter | Phospholipid metabolism |
| CLN2 | YPL256C | G1/S-specific cyclin, interacts with Cdc28 protein kinase to control events at START | Cell cycle progression |
| CDH1 | YGL003C | Cell cycle-regulated activator of the anaphase-promoting complex required for exit from mitosis | Cell cycle progression |
| WHI2 | YOR043W | Protein involved in stress response and growth regulation; negative regulator of G1 cyclin expression | Cell cycle progression/stress response |
| RIM9 | YMR063W | Protein of unkonwn function; involved in proteolytic activation of Rim101 in response to pH | Stress response |
| TUS1 | YLR425W | GDP-GTP exchange factor that functions to modulate Rho1 activity as part of the cell integrity pathway | Stress response/cell wall organization |
| SLG1 | YOR008C | Plasma membrane protein required for maintenance of cell wall integrity and for the stress response | Cell wall organization |
| ECM33 | YBR078W | Glycosylphosphatidylinositol anchor protein of unknown function; possible role cell wall organization | Cell wall organization |
| NCS2 | YNL119W | Role in urmylation | Cell polarity |
| NCS6 | YGL211W | Protein of unknown function; role in urmylation and invasive/pseudohyphal growth | Cell polarity |
| PEA2 | YER149C | Coiled-coil polarisome protein | Cell polarity |
| CRN1 | YLR429W | Coronin, actin and microtubules associated protein | Actin patch assembly |
| ICE2 | YIL090W | Protein of unknown function; integral endoplasmic reticulum membrane protein | Endoplasmic reticulum organization |
| PER1 | YCR044C | Vacuolar membrane protein; mutant is dependent on activation of the unfolded protein response | Protein Processing |
| DSD1 | YGL196W | d-Serine dehydratase | Amino acid metabolism |
| HXT8 | YJL214W | PM protein with strong similarity to hexose transporters; expression induced by low glucose | Hexose transport |
| LSM1 | YJL124C | Protein involved in mRNA degradation in cytoplasm | mRNA catabolism |
| CBF1 | YJR060W | Helix-loop-helix protein that binds to promoters in CDE1 and MET genes; required for chromosome stability | DNA/chromosome organization |
| RAD27 | YKL113C | Single-stranded DNA endonuclease and 5′-3′-exonuclease, 5′-flap endonuclease | DNA/chromosome organization |
| EAF7 | YNL136W | Subunit of the NuA4 histone acetyltransferase complex | DNA/chromosome organization |
| RAD34 | YDR314C | Nucleotide excision repair | DNA repair |
| YSA1 | YBR111C | Nucleosidediphosphate sugar hydrolase | Unknown |
| CUE3 | YGL110C | Protein of unknown function; has CUE domain that may facilitate intramolecular monoubiquitination | Unknown |
| YFR045W | YFR045W | Protein with similarity to mitochondrial transporter family | Unknown |
| YMR003W | YMR003W | Protein of unknown function | Unknown |
| YGR064W | YGR064W | Protein of unknown function | Unknown |
| YPL261C | YPL261C | Protein of unknown function | Unknown |
| YJL007C | YJL007C | Protein of unknown function | Unknown |
| YMR052C-a | YMR052C-a | Protein of unknown function | Unknown |