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. 2009 Mar 13;284(11):6627–6638. doi: 10.1074/jbc.M808779200

FIGURE 3.

FIGURE 3.

Effect of YoeB on cellular mRNAs and identification of YoeB cleavage sites in the chromosomally encoded lpp and ompA mRNAs. Total cellular RNA was extracted from E. coli BW25113 cells containing pBAD-YoeB at various time points as indicated after the addition of arabinose and subjected to Northern blot analysis using radiolabeled lpp and ompA open reading frames as probes shown in A and B, respectively. For in vivo primer extension, the lpp and ompA mRNAs were prepared from E. coli BW25113 cells containing pBAD-YoeB at various time points as indicated in C and D before and after the induction of YoeB. The sequence ladders for lpp and ompA were obtained using pCR®2.1-TOPO®-lpp and pCR®2.1-TOPO-ompA as template, respectively. The sequences around the major cleavage sites are shown at the right-hand side and the major primer extension stop sites are indicated by arrowheads. On the top of the gels, the full-length (FL) RNA bands are shown with an arrowhead and FL. C, primer extension analysis of the lpp mRNA. D, primer extension analysis of the ompA mRNA. FL, full-length.