TABLE 1.
MICs of aminoglycoside antibiotics for E. coli JM83 producing various APH(2″) enzymes
KanA, kanamycin A; KanB, kanamycin B; Gen, gentamicin; Tob, tobramycin; Sis, sisomicin; Ne, netilmicin; Dbk, dibekacin; Amk, amikacin; Arb, arbekacin; Ise, isepamicin; Neo, neomycin B; Par, paromomycin.
|
Enzyme
|
MIC
|
||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| KanA | KanB | Gen | Tob | Sis | Net | Dbk | G418 | Amk | Arb | Ise | Neo | Par | |||||||||||||
| μg/ml | |||||||||||||||||||||||||
| Ia | 256 | 32 | 32 | 16 | 16 | 4 | 32 | 512 | 2 | 1 | 1 | 1 | 4 | ||||||||||||
| Ib | 128 | 64 | 16 | 32 | 8 | 8 | 64 | 512 | 2 | 1 | 1 | 0.5 | 4 | ||||||||||||
| Ic | 16 | 64 | 64 | 128 | 32 | 4 | 256 | 128 | 1 | 0.5 | 1 | 0.5 | 4 | ||||||||||||
| Id | 16 | 16 | 32 | 16 | 8 | 2 | 16 | 128 | 1 | 0.5 | 1 | 0.5 | 4 | ||||||||||||
| Controla | 2 | 1 | 0.25 | 0.5 | 0.25 | 0.25 | 0.5 | 2 | 1 | 0.5 | 1 | 0.5 | 4 | ||||||||||||
a
Parental E. coli JM83 strain harboring plasmid pBluescript II KS(+) vector, which does not contain any genes for APH(2″) enzymes.