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Visible absorption spectra of T. cruzi Complex II. Purified Complex II was desalted by ultrafiltration and diluted with 0.1 m sodium phosphate, pH 7.2, containing 0.1% SML at a final concentration of 0.06 mg/ml. Absorption spectra of the air-oxidized (Ox, thin line) and dithionite-reduced (Rd, thick line) forms were recorded at room temperature with UV-2400 spectrophotometer (Shimadzu Corp., Kyoto, Japan).
