FIGURE 2.

The PPPPSPT motif is crucial for Zbed3 interacting with Axin. A, alignment of homologous motif in mouse Zbed3 and LRP5/6. Amino acid numbers of these motifs are shown. B, GSK3β and CKIε synergistically enhanced Zbed3 and Axin interaction. 24 h after transfection, 293T cells were subjected to co-immunoprecipitation and Western blotting as shown. C, Zbed3 (wild-type, WT), with LiCl (25 mm, 1-h treatment before lysing the cell) or its mutants (ΔM, SA,or TA) exhibited different interacting affinities in binding to Axin and (D) in the presence of co-transfected GSK3β and CKIε. Experiments were done as described in B.