FIGURE 1.

PKA activation modulates CYP3A gene expression in primary cultures of mouse and rat hepatocytes. Primary rodent hepatocytes were treated with 10 μm PCN and increasing concentrations of 1 mm 8-Br-cAMP for 24 h before RNA isolation. Blots were probed sequentially with ³²P-labeled fragments of CYP3A and 18 S.