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hPXR is phosphorylated by protein kinases in vitro. HepG2 cells were transduced with adenovirus expressing FLAG-tagged human PXR. A, human PXR was isolated from cellular extracts using immunoprecipitation with the M2 FLAG monoclonal antibody (Ab) and detected using SDS-PAGE and Coomassie staining. B, the recombinant human PXR protein was phosphorylated with γ-32P and myriad kinases in vitro. Radiolabeled proteins were visualized by autoradiography.
