FIGURE 8.

PKA increases the strength of interaction between hPXR and NCoR in mammalian-2-hybrid reporter gene assays. CV-1 cells were transfected with expression vectors for Gal4-NCoR receptor-interacting domain, VP16-human PXR, pFR-Luc reporter gene, and constitutively active kinases. Cells were treated with 10 μm rifampicin for 24 h. Data are expressed as the mean of replicates ± S.D. (n = 8) and are normalized to β-galactosidase activity. Letters different from each other indicate a statistical difference between treatment groups (p < 0.05).