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. 2009 Mar 13;284(11):6893–6908. doi: 10.1074/jbc.M805861200

FIGURE 3.

FIGURE 3.

L. major infection induces proteolysis of p130Cas and PTP-PEST. A, fibroblasts positive and negative for PTP-PEST expression were starved for 16 h in 0.05% heat-inactivated FBS/DMEM and incubated in starvation medium without or with L. major at a ratio of 1:20 (cells:parasites) for the indicated duration. IB, immunoblot. B, PTP-PEST-/- cells expressing WT PTP-PEST (clone B11WT) were exposed to different ratios (cells:parasites) of L. major for 10 min. C, PTP-PEST-/- cells rescued with either empty vector (clones B14V and B15V) or with the WT enzyme (clones B11WT and B118WT) were maintained in starvation or regular media (nonstarved) 16 h prior to treatment. Indicated clones were incubated with or without L. major in the absence of serum or with media supplemented with 10% heat-inactivated-FBS. Cell lysates were prepared and analyzed by immunoblotting for p130Cas and PTP-PEST. Filled arrowheads identify intact proteins, and open arrowheads point to cleavage products. L.m, L. major; SS, serum-starved; NS, nonstarved or in presence of serum. The values on the right correspond to molecular mass in kDa.