FIGURE 2.

Overall structure of GLNBP. The TIM barrel fold domain (blue), Ig-like fold domain (green), α/β fold domain (yellow), and C-terminal domain (red) are shown. A, dimeric structure of GlcNAc-NO₃-EG crystal form. Subunits in the closed and semiclosed states are shown in a ribbon model. The semiclosed state subunit is shown with a different color code (TIM barrel domain in light blue, Ig-like domain in light green, α/β domain in brown, and C-terminal domain in pink). The ligand-free form in the open state (subunit A) (B) and GlcNAc-NO₃-EG in the closed state (subunit A) (C) are shown from the same view as A. Ligands in the active site (GlcNAc, ethylene glycol, and ) are shown as a space-filling model. Asp-313 and Trp-233 are shown as a stick model (cyan), and the Cα atom of Gly-371 is shown as a red sphere.