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. 2009 Mar 13;284(11):7078–7090. doi: 10.1074/jbc.M806605200

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Copyright © 2009, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Calcium-binding loops of S100A9 and epithelial cell resistance to Listeria and Salmonella invasion. KB-sham, KB-S100A8/A9_1–114, KB-S100A8/A9_E36Q, KB-S100A8/A9_E378Q, and KB-S100A8/A9_E36Q,E78Q were analyzed for bacterial invasion using an antibiotic protection assay. Monolayers were incubated with L. monocytogenes ATCC 10403S (A) or S. typhimurium ATCC 14028 (B) at an m.o.i. of 100:1 and 1:1, respectively, for 2 h. Each experiment was performed in triplicate wells. Values are means ± S.E. of viable intracellular bacteria, relative to KB-sham (100%), from at least three independent experiments. C, immunofluorescence staining for intracellular and extracellular Listeria in KB-sham, KB-S100A8/A9_1–114, KB-S100A8/A9_E36Q, KB-S100A8/A9_E78Q, and KB-S100A8/A9_E36Q,E78Q transfectants. Monolayers were incubated with L. monocytogenes for 2 h. The intracellular bacteria were enumerated and reported as means ± S.E. relative to KB-sham (100%) as in the legend of Fig. 5. The results shown are from three independent experiments (^**, p < 0.01; #, p < 0.001).