FIGURE 3.
Binding of DgkB to PtdGro vesicles. A, DgkB binding to PtdGro vesicles determined by the AlphaScreen assay. Measurements were made in triplicate and the mean ± S.E. are plotted. The apparent Kd was calculated as 34 ± 1 nm. B, DgkB binding to PtdGro vesicles determined from the difference in the recovery of soluble DgkB treated with PtdCho vesicles compared with the recovery of soluble DgkB in the presence of PtdCho plus PtdGro vesicles as measured by densitometry of stained SDS gels. The apparent Kd using this assay was 760 ± 120 nm. Details of these two assays are found under “Experimental Procedures.”