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. 2009 Mar 13;284(11):7294–7306. doi: 10.1074/jbc.M806816200

FIGURE 11.

FIGURE 11.

1400W partially reverses the inhibition of amiloride-sensitive Na+ currents by rRA2 infection in GFP(+) cells. A, nitrite concentration of rRA2 infected (m.o.i. = 1) cells as measured by chemiluminescence 48 h after rRA2 infection. Cells were treated either by vehicle (0.1% DMSO; solid bars) or 1400W (1 μm; diagonal lines). Nitrite concentrations were normalized to those of the DMSO-treated cells. Values are the means ± 1 S.E., n = 6 from 3 independent experiments (**, p < 0.01, m.o.i. = 1). B, mean values ± 1 S.E. of amiloride-sensitive currents of GFP(+) cells at -60 mV incubated with either DMSO (solid bars, n = 4) or 1400W (diagonal lines, n = 5) 3 days after rRA2 infection. C and D, α and β ENaC mRNA levels in non-inoculated (solid bars), GFP(-)(diagonal lines), and GFP(+)(cross-hatched lines) cells (m.o.i. = 1) treated with DMSO or 1400W (1 μm). ENaC mRNA levels were measured by real-time RT-PCR from cells sorted and collected by flow cytometry 3-5 days after rRA2 infection and normalized by dividing them by those of the non-inoculated cell groups. Values are the means ± 1 S.E.; data are from 4 independent experiments (**, p < 0.01 compared with non-inoculated cells).