FIGURE 12.

Incubation of H441 cells with both A77-1726 and 1400W completely reverses the inhibition of amiloride-sensitive Na⁺ currents by rRA2 infection. H441 cells were preincubated with either vehicle (0.1% DMSO) or a combination of A77-1726 (20 μm) and 1400W (1 μm) for 12 h, then infected with rRA2 (m.o.i. = 1). DMSO or A77-1726 and 1400W were presented until whole-cell patch clamp recording 72 h after infection. Values are the means ± 1 S.E. Plotted are current densities at -60mV. GFP(+) cells incubated with DMSO exhibited significantly lower basal (solid bars) and amiloride-sensitive Na⁺ currents (diagonal lines) as compared with those of the non-inoculated cells (^*, p < 0.05; ^**, p < 0.01, n = 7). Treatment with A77-1726 and 1400W restored the basal and amiloride-sensitive currents of GFP(+) cells to near control levels (n = 7).