FIGURE 2.

Infection of H441 cells with rRA2 does not increase apoptosis. A, representative flow cytometry records of H441 cells not incubated with annexin V-PE serving as background. Shown is annexin V-PE binding to control (non-inoculated) H441 cells treated with 10 μg/ml actinomycin D for 48 h (B), non-inoculated cells (C) or cells infected with rRA2 for 6 days (D). Numbers of cells in P2 and P3 indicate annexin V-PE-negative and -positive cells, respectively. E, flow cytometry analysis of the percentage of annexin V-PE-positive cells in non-inoculated H441 cells (solid), cells infected with rRA2 (stripes at 45 degrees), infected with wild type RSV strain A2 (horizontal stripes), and UV-inactivated rRA2 (cross-hatched pattern) at different times after infection. Floating and adherent cells were pooled together; bars are the means ± 1 S.E.; data are from at least 4 samples from two independent experiments (all at m.o.i. = 1).