FIGURE 4.
Infection with rRA2 decreases Na+ single channel NPo in GFP(+) H441 cells. A, characteristic single channel Na+ current traces and amplitude histogram of a non-inoculated H441 cell patched in the cell-attached mode. Only a small portion of the entire record (∼10 min of recording) is shown. B, expanded view of the indicated portion of the record. The closed and open states of a channel are shown. The patch potential was approximately -100 mV (see “Materials and Methods”). Channel conductance was about 4 picosiemens, characteristic of ENaC. C, all events histogram for a 10-min recording showing the number of events (y axis) versus current in pA (x axis). D-F, characteristic single channel Na+ current traces and amplitude histogram of a GFP(+) cell 4 days after infection. Notice markedly decreased number of events in the all events histogram. G, NPo values of non-inoculated and GFP(+) cells. Values are the means ± S.E. (**, p < 0.01, n = 6 for control non-inoculated cells and 5 for GFP(+) cells, m.o.i. = 1).