FIGURE 9.

Infection of H441 cells with rRA2 up-regulates NF-κB activity. Western blots of p65 subunits of non-inoculated H441 cells (non-inoc.) or infected with either rRA2 or UV-inactivated rRA2 (m.o.i. = 1) for either 24 (A) or 48 h (B). Nuclear extracts were separated by SDS-PAGE and immunoblotted with antibodies to NF-κB p65 subunit. Each lane represents a different experiment. C, EMSA gel image of non-inoculated cells or cells infected with rRA2 for either 24 or 48 h (m.o.i. = 1). Infection with rRA2 increased NF-κB activity at either time point. Results of a typical experiment which were repeated two times.