Table 1. Transfection Efficiencies of C60/DNA Transfection Mixtures at Various R Values Presented as a Quotient of GFP-Fluorescent Cells Relative to the Total Number of Cells Analyzed as Measured by Flow Cytometrya.
transfection efficiency (%) at various R values |
|||||
---|---|---|---|---|---|
C60 vector | R = 0.42 | R = 4.2 | R = 8.4 | R = 16.8 | R = 42 |
IV | 0.53 ± 0.13 | 0.42 ± 0.18 | 1.32 ± 0.57 | 31.33 ± 4.24 | 2.40 ± 0.13 |
III | 0.43 ± 0.31 | 0.37 ± 0.21 | 0.99 ± 0.15 | 28.33 ± 4.94 | 1.86 ± 0.24 |
II | 0.30 ± 0.20 | 0.58 ± 0.14 | 0.60 ± 0.13 | 0.32 ± 0.06 | 0.41 ± 0.17 |
I | 0.27 ± 0.15 | 0.45 ± 0.28 | 0.62 ± 0.10 | 0.34 ± 0.04 | 0.43 ± 0.14 |
V | 0.17 ± 0.12 | 0.36 ± 0.27 | 0.23 ± 0.10 | 0.11 ± 0.03 | 0.09 ± 0.05 |
VI | 0.27 ± 0.21 | 0.15 ± 0.06 | 0.48 ± 0.25 | 0.10 ± 0.01 | 0.14 ± 0.04 |
naked DNA | 0.01 ± 0.005 | ||||
PEI (25 kDa) | 28.7 ± 3.5 | ||||
Cytopure | 35.2 ± 5.1 |
10,000 events were counted for each sample. C60 vectors are listed in order of their measured efficiency at promoting transfection in NIH 3T3 cells under serum-free conditions.