Table 2. Comparison of Transfection Efficiencies for Octa-Amino C60 Compound III and Dodeca-Amino C60 Compound IV at R = 4.2 and R = 16.8 for NIH 3T3 Cells, HEK 293 Cells and MSCs under Serum-Free Transfection Conditionsa.
transfection efficiencies (%) |
||
---|---|---|
cell line studied at distinct R-values | III/DNA | IV/DNA |
NIH 3T3 (R = 4.2) | 0.37 ± 0.21 | 0.42 ± 0.18 |
NIH 3T3 (R = 16.8) | 28.33 ± 4.94 | 31.33 ± 4.24 |
HEK 293 (R = 4.2) | 0.54 ± 0.08 | 0.60 ± 0.10 |
HEK 293 (R = 16.8) | 7.66 ± 0.22 | 10.05 ± 0.03 |
MSC (R = 4.2) | 0.09 ± 0.03 | 0.39 ± 0.03 |
MSC (R = 16.8) | 0.05 ± 0.01 | 0.07 ± 0.02 |
Transfection efficiency values are presented as a quotient of GFP (+) cells relative to the total viable cells present in the transfected sample well as measured by flow cytometry. [10,000 events were counted for each sample.]