Table 1.
Silencing of HsRAD51 recombinase leads to decreased expression of several homologous recombination genes
| Sr. no. | GenBank accession no. | Gene name | Fold reduction in gene expression |
|---|---|---|---|
| 1 | NM_002 874.1 | RAD23 homolog B (S cerevisiae) | 11.5 |
| 2 | NM_885728.1 | Endonuclease G-like 2 (ENDOGL2) | 4.3 |
| 3 | AF035B2D.1 | BRCA1-associated protein | 3.7 |
| 4 | U79718.1 | nth endonuclease Ill-like 1 (E coli) | 2.2 |
| 5 | NMJ04111.3 | Flap structure–specific endonuclease 1 | 1.6 |
| 6 | NM_885732.1 | RAD58 homolog | 1.4 |
| 7 | NMJ0287B.1 | RAD51 homolog C | 1.4 |
| 8 | NM_884435.1 | Endonuclease G | 1.4 |
| 9 | NMJ05431.1 | XRCC2 | 1.3 |
Gene expression profile was measured in ARP myeloma cells after transfection with control siRNA or siRNA directed at HsRAD51 and RAD51B as described in “Methods.” Fold reduction in the expression of HR genes in cells transfected with RAD51 siRNA relative to control cells is shown.
HR indicates homologous recombination; and siRNA, small inhibitory RNA.