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. 2009 Feb 24;7(2):e1000040. doi: 10.1371/journal.pbio.1000040

Figure 3. Analysis of Tissue Patterning and Differentiation in Third Instar Eye Discs.

Figure 3

Immunohistochemistry was used for armadillo (arm, green), embryonic lethal, abnormal vision (elav, blue), senseless (sens, red), and diamidinophenylindole (dapi, grey)

(A) Wild-type eye disc.

(B) ey-GAL4, UAS-Dl/+ shows enlarged discs with wild-type patterning.

(C) ey-GAL4, UAS-Dl/UAS-atoERD show disrupted patterning with expansion of the undifferentiated domain (white arrows). Proliferative outgrowth is indicated with an open arrow.

(D) ey-GAL4, UAS-Dl, eyeful/+.

(E) Gain of ato function in the eyeful background leads to restoration of the pattern of differentiation: ey-GAL4, UAS-Dl, eyeful/UAS-ato shows almost normal appearance of all markers.

(F) Loss of ato function in an eyeful background leads to loss of uniform arm staining and a loss of and abnormal pattern of differentiation (elav and sens, white arrows). ey-GAL4, UAS-Dl, eyeful/UAS-atoERD.

Images and orthogonal sections are shown. All images were taken at the depth of the nuclei. Scale bars represent 100 μm.