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. 2009 Feb 24;7(2):e1000043. doi: 10.1371/journal.pbio.1000043

Figure 4. REV-ERBα and β-Mediated Repression Is Relieved by a NO Donor.

Figure 4

(A) Endogenous mRNA levels of Bmal1 and Rev-erbα and β in control and Rev-erbα and β siRNA treatments.

(B) Bmal1 and Rev-erbα and β gene expression following treatment with either 300 μM Deta/NO or 20 mM LiCl.

(C) Comparison of Bmal1 gene expression levels in control, Deta/NO- and siRNA-treated cells.

(D) Transient transfection of 293T cells with GAL4-REV-ERBα or GAL4-REV-ERBβ results in repression of the UASGAL4-containing luciferase reporter. Treatment of the cells with Deta/NO leads to a 3-fold derepression of luciferase expression. All experiments were performed in triplicate, with the error bars showing sample to sample variation. Experimental data shown are representative of a minimum of three repeats under the same conditions.