Table 1. Results obtained during Microchecker analyses.

Loci with positive F IS	Observed blanks	Total sample size	Expected blank frequency
			Brookfield 2	P-value	Males	P-value
pGp24	54	192	0.315	0.179	ND	ND
A10	3	30	0.149	0.329	ND	ND
pGp1	1	30	0.032	0.754	ND	ND
X55.3	9	109	0.045	0.975	0.039	0.989
XpGp11	19	109	0.137	0.893	0.026	1
XB110	3	109	0.033	0.516	0.076	0.030

The six loci that gave positive results are presented with the total number of genotyped individuals for each locus (blanks included). The sum of expected blanks over all samples divided by the sample size gave the expected frequency of blanks and the result of the exact binomial test comparing the observed proportion of blanks to the expected one (H1: there are less blanks than expected with null alleles). Expected frequencies were computed with Brookfield's second method for all loci or directly with the proportion of blank males at X-linked loci as described in the Methods section. Significant test (in bold) means that not enough blank individuals were observed at that locus for null alleles to explain the observed F _IS.