Table 1.

Primer sequences used for PCR amplification

Amplified region	Primer	Sequence	Product size (bp)
promoter region (950 bp) and 5' part of rpoB gene (721 bp)	P-rpo-s	5'-tctagacgagagcggcggtgcaatc	1671
	P-rpo-r	5'-gctcgctggtccagcccagc
3' part of rpoB gene (1258 bp) and downstream region (218 bp)	3'rpo-s	5'-cgacaccaagctgggtgcgg	1476
	3'rpo-r	5'-aagcttccagtcgcgagtcggcccg
BstEII fragment of rpoB gene including 81-bp hot spot region	bst-s	5'-cgcgacaccgtcggcgtgcg	1852
	bst-r	5'-aagtgtcgcgcacctcgcgggc
pMV306 (221 bp) and insert DNA cloned in MCS of this vector	MV-r	5'-aaggcccagtctttcgactgagc	221 + insert
	MV-s	5'-gtggataaccgtattaccgcc	DNA