Table 2.
Plasmids used in this study
| Plasmid | Description | Source |
|---|---|---|
| Cloning vectors | ||
| pGemTEasy | T/A cloning | Promega |
| pMV306H | mycobacterial integrating vector, HygR | Med-Immune Inc. |
| pMV306K | mycobacterial integrating vector, KanR | Med-Immune Inc. |
| pMV261 | mycobacterial Escherichia coli shuttle vector, carrying heat shock (Phsp65) promoter, KmR | Med-Immune Inc. |
| RpoB expression vectors | ||
| pMRP1 | wild type rpoB of M. tuberculosis H37Ra controlled by natural promoter PrpoB cloned in integration vector pMV306K, KanR | This study |
| pMRP2-9 | mutated rpoB of M. tuberculosis clinical strains controlled by natural promoter PrpoB cloned in integration vector pMV306K; 2-represents H526D; 3-D516V; 4-Q510H/D516Y; 5-S512I/D516G; 6-Q513L; 7-M515I/D516Y; 8-D516Y; 9-S531L, respectively, KanR | This study |
| pMERP1 | wild type rpoB of M. tuberculosis H37Ra controlled by heat shock promoter Phsp65 in pMV261, KanR | This study |
| pMERP2-9 | mutated rpoB of M. tuberculosis clinical strains controlled by heat shock promoter Phsp65 in pMV261, 2-represents H526D; 3-D516V; 4-Q510H/D516Y; 5-S512I/D516G; 6-Q513L; 7-M515I/D516Y; 8-D516Y; 9-S531L, respectively, KanR | This study |
| pMHRP1 | wild type rpoB of M. tuberculosis H37Ra controlled by heat shock promoter Phsp65 in pMV306, HygR | This study |
| pMHRP2-9 | mutated rpoB of M. tuberculosis clinical strains controlled by heat shock promoter Phsp65 in pMV306, '2-represents H526D; 3-D516V; 4-Q510H/D516Y; 5-S512I/D516G; 6-Q513L; 7-M515I/D516Y; 8-D516Y; 9-S531L, respectively, HygR | This study |