Table 4.
Order | Primer name | Sequence (5'-3') | Length | position | Product size (bp) |
1* | HK-4343F | TTAGAGTTCCGTTTCACCCG | 20 | 4343 | 2470, 4617 |
HK-6812R | CTTTCGCTTCAATTTAGTTAGT | 22 | 6812, 8959 | ||
2* | HK-6569F | GGTTCTGGTATAATGTTAGCT | 21 | 6569, 8716 | 824, 2971 |
HK-7392R | ATTACTCTCTTTTTACTCCC | 20 | 7392, 9539 | ||
3* | HK-9412F | CTAGGTCAGGTCGAAGTGCT | 20 | 7265, 9412 | 1016, 3163 |
HK-10427R | AGAGCACAGGTGTTGGGTGA | 20 | 10427 | ||
4 | HK-5807F | GTCTCATAATCCGAAAGTGGTT | 22 | 5807 | 2658 |
HK-8464R | CTTATACTTGGGCTACTTTCTT | 22 | 8464 | ||
5 | HK-8138F | GGTGCTCACTAAATCAGTATGT | 22 | 8138 | 1905 |
HK-10043R | ATGAAGATAGTGACGGAAACCC | 22 | 10043 |
*Primer pairs from Yu et al. (2008). Because one or both of the primers are located in the duplicated rrnS gene, two fragments are expected but only the shorter one is actually amplified.