. 2008 May 13;295(2):E323–E330. doi: 10.1152/ajpendo.00024.2008

Table 2.

Expression of mediators of cardiac dysfunction associated with HF diet in C57Bl6J and RAGE KO mice, measured by real time RT-PCR

Gene Expression (AU/18 s)	Wild Type			RAGE KO
Gene Expression (AU/18 s)	SD	HF	HF + AL	SD	HF	HF + AL
RAGE	1.0±0.2	2.5±0.4^*	1.0±0.2^#	ND	ND	ND
Hypertrophy
α-MHC	1.0±0.2	1.0±0.1	0.7±0.2^*	0.8±0.1	1.0±0.2	0.6±0.2^*^#
β-MHC	1.0±0.2	3.2±0.5^*	2.5±0.3^*	0.9±0.2	1.3±0.3^#	1.6±0.2^*^#
Inflammation
Il-6	1.0±0.3	18.0±5.3^*	0.2±0.1^*^#	0.9±0.2	0.3±0.1^*^#	0.4±0.1^*^#
TNF-α	1.0±0.2	2.0±0.3^*	0.5±0.2^*^#	1.4±0.3	0.3±0.1^*^#	0.4±0.1^*^#
ICAM-1	1.0±0.1	5.6±0.8^*	0.2±0.1^*^#	1.0±0.2	0.6±0.1^*^#	0.2±0.1^*^#
MCP-1	1.0±0.1	3.0±0.6^*	0.4±0.1^*^#	1.0±0.1	0.7±0.2^*^#	0.2±0.1^*^#
p65	1.0±0.1	1.5±0.2^*	0.8±0.2^*^#	0.5±0.1^*	0.5±0.1^*^#	0.3±0.1^*^#
Fibrosis
Collagen I	1.0±0.1	1.6±0.1^*	1.6±0.1^*	0.4±0.1^*	0.7±0.1^*^#	0.8±0.1^#
Collagen IV	1.0±0.1	1.3±0.1^*	1.3±0.1^*	0.6±0.1^*	0.7±0.1^*^#	0.9±0.1^#
Oxidative stress
gp91^phox	1.0±0.1	1.8±0.3^*	0.8±0.2^#	1.0±0.1	1.0±0.1	0.9±0.1
RAC-1	1.0±0.1	1.0±0.1	1.0±0.2	0.7±0.1^*	0.7±0.1^*	0.7±0.1^*
HO	1.0±0.2	0.2±0.1^*	0.1±0.1^*	1.0±0.2	0.2±0.1^*	0.1±0.1^*
Metabolic
PPARα	1.0±0.2	3.0±0.5^*	1.9±0.4^*^#	1.0±0.2	3.0±0.6^*	1.2±0.3^#

Values are means ± SE. MHC, myosin heavy chain; ICAM, intercellular adhesion molecule; MCP, macrophage chemoattactant factor; HO, heme oxygenase; ND, not detected.

Significant difference vs. wild-type SD;

vs. wild-type HF, P < 0.05.