Table 3.
Offspring body weight expressed as LS mean (g) ± LS-SEM (percent reduction) from P21−98. P56 was chosen to show body weight shortly before the start of behavioral testing. P98 was chosen to show body weight at the conclusion of behavioral testing.
| Regimen | Sal | MA10 | MA25 |
|---|---|---|---|
| P1−10 | Males | ||
| 21a | 62.6 ± 1.7 | 45.0 ± 1.8** (27.3%) | 36.7 ± 2.0** (41.4%) |
| 56b | 364.3 ± 6.9 | 321.9 ± 7.2** (11.6%) | 314.0 ± 8.4** (13.8%) |
| 98b | 527.1 ± 6.9 | 477.6 ± 7.2** (9.4%) | 491.2 ± 8.4** (6.8%) |
| N | 21 | 19 | 14 (10)c |
| P6−15 | |||
| 21a | 65.8 ± 1.7 | 44.4 ± 1.7** (32.5%) | 35.3 ± 1.7** (46.4%) |
| 56b | 357.6 ± 6.9 | 326.9 ± 7.1** (8.6%) | 298.9 ± 6.5** (16.4%) |
| 98b | 532.4 ± 6.9 | 487.6 ± 7.1** (8.4%) | 460.1 ± 6.5** (13.6%) |
| N | 21 | 20 | 26 (17)c |
| P11−20 | |||
| 21a | 62.9 ± 1.8 | 42.8 ± 1.8** (32.0%) | 35.3 ± 1.7** (43.9%) |
| 56b | 358.7 ± 7.3 | 343.0 ± 7.3 (4.4%) | 321.3 ± 6.4** (10.4%) |
| 98b | 526.8 ± 7.3 | 523.4 ± 7.4 (0.6%) | 521.8 ± 6.5 (0.9%) |
| N | 19 | 19 | 28 (17)c |
| P1−10 | Females | ||
| 21a | 60.7 ± 1.7* | 45.4 ± 1.7** (25.2%) | 38.2 ± 2.1** (37.1%) |
| 56b | 232.4 ± 6.9 | 218.6 ±7.1 (5.9%) | 206.3 ± 8.9* (11.2%) |
| 98b | 312.5 ± 6.9 | 296.0 ± 7.1 (5.3%) | 277.2 ± 9.1* (11.3%) |
| N | 21 | 20 | 11 (10)c |
| P6−15 | |||
| 21a | 61.6 ± 1.7 | 46.2 ± 1.7** (25.0%) | 36.0 ± 1.6** (41.6%) |
| 56b | 229.8 ± 7.1 | 219.0 ± 6.9 (4.7%) | 201.6 ± 5.9** (12.3%) |
| 98b | 304.1 ± 7.1 | 301.4 ± 6.9 (0.9%) | 284.7 ± 5.9* (6.4%) |
| N | 20 | 21 | 34 (20)c |
| P11−20 | |||
| 21a | 58.2 ± 1.8 | 41.8 ± 1.8** (28.2%) | 35.8 ± 1.6** (38.5%) |
| 56b | 229.3 ± 7.3 | 226.7 ± 7.3 (1.1%) | 213.8 ± 6.0 (6.8%) |
| 98 | 313.7 ± 7.9 | 307.5 ± 7.4 (2.0%) | 301.6 ± 6.1 (3.9%) |
| N | 19 | 18 | 33 (18)c |
P21 and 28 (preweaning) body weights were analyzed together. Significant effects were the group main effect (F(2,316) =995.13, p < 0.0001), sex main effect (F(1,312) = 34.61, p < 0.0001), day main effect (F(1,370) = p < 0.0001), group × sex interaction (F(2,312) = 15.92, p < 0.0001), group × regimen (F(4,315) = 4.11, p < 0.003), group × day interaction (F(2,370) = 15.96, p < 0.0001), sex × day (F(1,370) = 73.52, p < 0.0001), group × sex × week (F(2,370) = 5.32, p < 0.01, regimen × day (F(2,370) = 20.9, p < 0.0001), and group × regimen × day (F(4,370) = 4.92, p < 0.001. No other effects were significant.
P35−98 (postweaning) body weights were analyzed together. Significant effects were the group main effect (F(2,321) = 76.82, p < 0.0001), sex main effect (F(1,315) = 3342.84, p < 0.0001), day main effect (F(9,3267) = 8738.13, p < 0.0001), group × regimen interaction (F(4,320) = 5.29, p < 0.001), sex × day interaction (F(9,3268) = 1114.35, p < 0.0001), regimen × day (F(18,3267) = 6.77, p < 0.0001), and group × regimen × day (F(36,3268) = 1.46, p < 0.04). No other effects were significant. When the latter interaction was further sorted with separate analysis on P56 and P98, both MA-treated groups showed weight reductions at the beginning of behavioral testing but these dissipated between P56−98 and became non-significant the MA10 group in all 3 regimens for females but remained significantly reduced in males for the P1−10 and P6−15 regimens. For the MA25 groups body weight reductions remained longer and did not catch up to Saline controls in the P1−10 or P6−15 regimens for males, but did for the P11−20 males. MA25 females showed catch up in both the P6−15 and P11−20 regimens.
Number in parentheses represents the number of animals remaining for behavioral testing after culling in the MA25 group which had extra animals assigned to it. One or both of these extra animals were included if the litter was short a high dose animal because of mortality but removed if the original male or female in the litter survived.
p < 0.05 vs. Sal
p< 0.001 vs. Sal