Figure 2.

Changes in expression of HvABA8′OH1 (A), HvNCED1 (B) and HvNCED2 (C) in embryos of whole grains (white bars) or of isolated embryos (grey bars) incubated on water and whole grains placed on GA₃ 1 mM (hatched bars) at 30°C. Data from real-time PCR (mean of 4 replicates ± SD) were normalised with actin transcript abundance and were expressed as percentage of the values obtained in embryos isolated from dry seeds. Absolute transcript amounts were respectively 942.1 ± 143.8, 81.8 ± 13.9 and 42.1 ± 5.7 copies per microliter of cDNA solution after 50 times dilution for HvABA8′OH1, HvNCED1 and HvNCED2 before imbibition.