Figure 1.

ins2 transposon in maize Y1 pushes away light-responsive cis-acting elements. (A) light-responsive cis-acting elements within maize Y1 and y1-602C allele, sorghum and rice PSY1 5′ upstream regions were predicted with PlantCARE (http://bioinformatics.psb.ugent.be/webtools/plantcare/html). Transposon ins2 inserts 300 bp upstream of transcript starting site in maize Y1 but not in allele y1-602C. (B) light-induced PSY1 and PSY2 expresssion during de-etiolation in y1-602C allele. Dark-grown nine-day-old dark-grown maize y1-602C seedlings were illuminated with white light (50 µmol m⁻² s⁻¹) for 0 to 8 h. The leaves of illuminated seedlings were harvested for cDNA preparation and used as templates for quantitative RT-PCR as carried out previously.²⁰ All quantifications were first normalized to β-actin amplified using the same conditions and were made relative to PSY1 transcript levels in unilluminated seedlings. Values represent the mean of three RT-PCR replicates ±SD from five pooled plants.