Figure 1. Distribution of microglia in the retina.
(A) Agarose-embedded sections showing distribution of GFP-positive microglia (in green) in CX3CR1+/GFP animals in the inner retina, primarily in the ganglion cell layer (GCL), inner plexiform layer (IPL), and outer plexiform layer (OPL). Nuclei were labeled with DAPI (blue), and vessels with GSIB4 lectin (red) (B) Confocal image from a retinal wholemount explant showing the distribution of ramified, GFPpositive microglia in the outer plexiform layer. (Scale bar = 50 µm) INL = inner nuclear layer, ONL = outer nuclear layer, OS = photoreceptor outer segments.