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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Mol Microbiol. 2008 Jul 9;69(5):1290–1303. doi: 10.1111/j.1365-2958.2008.06362.x

Figure 7. Overexpression of exoS increases the amount of ExoS associated with ExoR.

Figure 7

All strains have chromosomal exoS tagged with myc. In addition, strains contain pRF771 vector only (lane 2) or myc-exoS expressed from the constitutive trp promoter in pRF771 (pEC491; lanes 1 and 3). exoR is expressed from its native promoter in the chromosome and is either untagged (lane 1) or V5-tagged (lanes 2 and 3). Anti-V5 immunoprecipitates (A) or total cell lysates (B) from EC457 (lane 1), EC475 (lane 2), and EC476 (lane 3), were subjected to SDS-PAGE and western blotting with either anti-myc polyclonal or anti-V5 monoclonal antibody. Arrowheads indicate mature ExoR. The faint band in the anti-V5 western panel in lane 1 of (A) is the light chain of the antibody used for immunoprecipitation.