Table 3.
Symbiotic defects of supr mutations and suppression by chvI(K214T)
| Strain | 15 days after inoculation |
29 days after inoculation |
|||
|---|---|---|---|---|---|
| Pink nodules (%) |
Total nodules per plant |
Pink nodules (%) |
Total nodules per plant |
Plant height (mm) |
|
| WT | 88 ± 4 | 5.6 ± 0.8 | 86 ± 4 | 6.3 ± 0.8 | 41 ± 4 |
| exoS(I87V) | 26 ± 8 | 2.6 ± 0.5 | 83 ± 7 | 3.6 ± 0.5 | 20 ± 3 |
| exoS(G268S) | 6 ± 4 | 3.6 ± 0.4 | 44 ± 9 | 4.9 ± 0.6 | 13 ± 2 |
| exoR(S156Y) | 54 ± 7 | 4.8 ± 0.5 | 64 ± 7 | 6.3 ± 0.6 | 34 ± 4 |
| exoR(G76C) | 17 ± 7 | 3.1 ± 0.6 | 59 ± 8 | 4.9 ± 0.6 | 17 ± 3 |
| chvI(K214T) | 11 ± 5 | 5.3 ± 0.5 | 39 ± 8 | 9.1 ± 0.7 | 13 ± 3 |
| chvI(K214T) exoS(I87V) | 69 ± 5 | 6.6 ± 0.7 | 67 ± 5 | 8.9 ± 0.6 | 35 ± 4 |
| chvI(K214T) exoS(G268S) | 68 ± 7 | 5.8 ± 0.5 | 79 ± 5 | 7.1 ± 0.5 | 47 ± 4 |
| chvI(K214T) exoR(S156Y) | 77 ± 6 | 5.0 ± 0.5 | 83 ± 4 | 6.2 ± 0.5 | 38 ± 3 |
| chvI(K214T) exoR(G76C) | 68 ± 8 | 5.1 ± 0.5 | 61 ± 7 | 7.1 ± 0.6 | 36 ± 4 |
Means and standard error are presented from 21 alfalfa seedlings inoculated for each strain (the same strains as in Fig. 3). Nodulation assays were performed twice with similar results; results from one of the experiments is shown.