(A) Samples of total RNA from control and 1 μM B[a]P-treated Swiss 3T3 cells were separated by electrophoresis on agarose gels (20μg/lane), transferred to nitrocellulose, and probed with various 32P-labelled cDNAs as indicated.
(B) Quiescent Swiss 3T3 cells were treated with 1 μM B[a]P or 10 nM TCDD for 24 hr. RNA prepared from the resulting cells was analyzed by RT-PCR using primers specific for Smoc2 and β-Actin as described under ‘Materials and Methods’.