Skip to main content
. Author manuscript; available in PMC: 2009 Mar 9.
Published in final edited form as: Drug Metab Dispos. 2005 Apr 8;33(7):937–946. doi: 10.1124/dmd.104.003061

Fig. 2.

Fig. 2

Analysis of stability of UGT2B7 mRNA in Caco-2 cells. Stability of UGT2B7 mRNA was analyzed by addition of actinomycin-D (1 μg/ml). Total RNA was extracted at various time points, and RT-PCR of UGT2B7 mRNA was performed. Plotted data are expressed as percentage of mRNA remaining and are representative of three independent experiments. The half-life of UGT2B7 mRNA in actinomycin-D-treated cells was calculated by fitting the percentage of mRNA remaining versus time data with a one-phase exponential decay equation using GraphPad Prism 4. (Y = span · e−K*X + plateau, where X = time, and Y = % mRNA remaining. Y = span + plateau at X = 0 and decreases to plateau with a rate constant, K. The half-life of the decay is 0.6932/K.)