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. Author manuscript; available in PMC: 2009 Sep 6.
Published in final edited form as: Science. 2009 Mar 6;323(5919):1354–1357. doi: 10.1126/science.1169218

Figure 4. Preferential adsorption of VM-GFP onto smaller vesicles is concentration dependent.

Figure 4

(A) Membrane fluorescence intensity as a function of vesicle diameter for increasing bulk concentrations of VM-GFP. At higher concentrations, vesicles with a diameter below a critical value (Dc) of ∼ 4 μm were preferentially labeled. (B) Membrane fluorescence intensity as a function of VM-GFP concentration for different vesicle diameters (indicated diameters are +/- 0.5 μm). Above Dc, all data points were similar and well approximated by a linear fit (dotted line), whereas below Dc, the isotherms were steeper and deviated from linear. (C) Typical behaviors for small (1.5 +/- 0.5 μm) and large (6.5 +/- 0.5 μm) vesicles, and theoretical curves obtained using a model for cooperativity (11). Data points are mean +/- SD measured on N vesicles (N=3-26), resulting from 3 independent experiments.