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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Cell Signal. 2009 Jan 19;21(5):793–800. doi: 10.1016/j.cellsig.2009.01.025

Figure 3. Constitutive Akt phosphorylation in EL4 cell lines.

Figure 3

In panel A, whole-cell extracts from WT2, V3, and V7 cells, equalized for protein, were immunoblotted for phospho-Akt and total Akt. In panel B, WT2 and V7 cells were incubated with 100 nM PMA for indicated times. Whole-cell extracts, equalized for protein, were immunoblotted for phospho-Akt and total Akt. In panel C, V7 cells were incubated with 50 μM LY294002 for indicated times. Whole-cell extracts, equalized for protein, were immunoblotted for phospho-Akt and total Akt.