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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Cell Signal. 2009 Jan 19;21(5):793–800. doi: 10.1016/j.cellsig.2009.01.025

Figure 6. Effects of U0126 on PMA-induced Raf and Erk phosphorylation in WT2 cells.

Figure 6

In panel A, WT2 cells were incubated in the absence and presence of 10 μM U0126 for the indicated times, and then treated with or without PMA for 15 minutes. Whole-cell extracts, equalized for protein, were immunoblotted for phospho-Erk and total Erk. In panel B, WT2 cells were incubated in the absence and presence of 10 μM U0126 for 2 hours, and then treated with PMA for the indicated times. Whole-cell extracts, equalized for protein, were immunoblotted for phospho-Raf (Ser259, Ser289/296/301, Ser338), phospho-Erk and total Erk.