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. Author manuscript; available in PMC: 2009 Mar 9.
Published in final edited form as: Eur J Immunol. 2006 Jun;36(6):1374–1385. doi: 10.1002/eji.200635883

Figure 3.

Figure 3

T-bet and Egr-2 were detected in the nuclear extracts of PI-Treg cells and associated with the induction of p27kip. Cytosolic and nuclear proteins were extracted from CD4 T cells from naive and tolerant mice before and after stimulation with Ac1-9[4Y] for 2 h. Western blots were stained with antisera specific for (A) Egr-2, p27kip and (B) T-bet, respectively, as indicated. The cytosolic and nuclear extracts in (B) are indicated as C and N. The results shown are representative of three separate experiments.